Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti STAT3 antibody or (B) Mouse anti STAT3 (pSer727) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000. Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 52 second or a (B) 300 second exposure. STAT3 (pSer727) has a molecular weight of 84 kDa.

Western Blot analysis of HepG2 untreated and EGF treated whole cell lysates probed with (A) Mouse anti STAT3 antibody (VMA00242) or (B) Mouse anti STAT3 (pSer727) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P). Membranes were treated with (+) and without (-) lambda protein phosphatase and visualized on the ChemiDoc MP with a (A) 52 second or a (B) 300 second exposure. STAT3 (pSer727) has a molecular weight of 84 kDa.